Essential Stem Cell Methods

ESSENTIAL STEM CELL METHODS




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Edited By
Robert Lanza, Chief Scientific Officer, Advanced Cell Technology, MA, USA; Adjunct Professor, Institute of Regenerative Medicine, Wake Forest University School of Medicine, NC, USA Robert Lanza Advanced Cell Technology 381 Plantation Street Worcester, MA 01605
Irina Klimanskaya, Advance Cell Technology, Worcester, MA, USA

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Contents




Section 1: Organ-Derived Stem Cells

1. Neural Stem Cell Isolation and Characterization ; Rodney L. Rietze and Brent A. Reynolds
2. Neural Stem Cells and Their Manipulation; Evan Snyder
3. Retinal Stem Cells; Thomas A. Reh and Andy J. Fischer
4. Dental Pulp Stem Cells; He Liu, Stan Gronthos and Songtao Shi
5. Spermatogonial Stem Cells; Jon M. Oatley and Ralph L. Brinster
6. Stem Cells in the Lung; Xiaoming Liu, Ryan R. Driskell and John F. Engelhardt
7. Pancreatic Cells and Their Progenitors; Seth J. Salpeter and Yuval Dor
8. Pluripotent Stem Cells from Germ Cells; Candace L. Kerr, Michael J. Shamblott and John D. Gearhart
9. Amniotic Fluid and Placental Stem Cells; Dawn M. Delo, Paolo De Coppi, Georg Bartsch, Jr. and Anthony Atala
10. Cord Blood Stem and Progenitor Cells; Mervin C. Yoder
11. Purification of Hematopoietic Stem Cells Using the Side Population; K.K. Lin and Margaret A. Goodell
12. Microarray Analysis of Stem Cells and Differentiation; Howard Y. Chang
13. Tissue Engineering Using Adult Stem; Cells Daniel Eberli
14. Mesenchymal Stem Cells and Tissue Engineering; Nicholas W. Marion and Jeremy J. Mao


Section II: Embryonic Stem Cells

15. Murine Embryonic Stem Cells; Andras Nagy and Kristina Vintersten
16. Human Embryo Culture; Amparo Mercader, Diana Valbuena and Carlos Sim n
17. Human Embryonic Stem Cells; Douglas Melton
18. Characterization and Evaluation of Human Embryonic Stem Cells; Chunhui Xu
19. Feeder-Free Culture of Human Embryonic Stem Cells; Michal Amit and Joseph Itskovitz-Eldor
20. Neural Stem Cells, Neurons, and Glia; Steven M. Pollard, Alex Benchoua and Sally Lowell
21. Hematopoietic Cells; Malcolm A.S. Moore, Jae-Hung Shieh and Gabsang Lee
22. Cardiomyocytes; Xiangzhong Yang, Xi-Min Guo, Chang-Yong Wang and X. Cindy Tian
23. Insulin-Producing Cells; Insa S. Schroeder, Gabriela Kania, Przemyslaw Blyszczuk
24. Transgene Expression and RNA Interference in Embryonic Stem Cells; Holm Zaehres and George Q. Daley
25. Lentiviral Vector-Mediated Gene Delivery into Human Embryonic Stem Cells; Michal Gropp and Benjamin Reubinoff
26. Engineering Embryonic Stem Cells with Recombinase Systems; Frank Schn tgen, A. Francis Stewart, Harald von Melchner and Konstantinos Anastassiadis
27. Tissue Engineering Using Embryonic Stem Cells; Shahar Cohen, Lucy Leshanski and Joseph Itskovitz-Eldor